Single Cell Isolation Microfluidics

We demonstrate the benefit of this approach to screen antibodies for the specific binding of cancer target cells in a high throughput single cell format which should have immense clinical potential.

Single cell isolation microfluidics. Here we present a microfluidic system that can overcome these limitations making use of a dual color signal normalization approach. 133 134 abvitro later acquired by juno therapeutics developed a similar approach for sequencing. Single cell analysis has become one of the most important applications of microfluidic technology in recent years. Using a hybrid microfluidics method droplets in channels can be individually addressed using a co planar electrode system.

The unique droplet microfluidic technology speeds up the process at the step of isolating activated single antibody secreting cells in picoliter volume chambers from various tissues including blood bone marrow and b cell plasma which allows secreted antibodies to be detected within hours with a variety of bead based and cell based assay formats. In this work a microfluidic method that is capable of isolating single cells in droplets selecting successful edited clones and expansion of these isoclones is introduced. Dielectrophoretic digital sorting enzymatic digestion facs hydrodynamic traps laser capture microdissection manual picking microfluidics micromanipulation serial dilution and raman tweezers. Methods currently used for single cell isolation include.

131 132 in parallel this technology has been developed commercially by gigagen. It refers to the process of isolation manipulation and analysis of the cells at single cell resolution in microfluidic chips.